- We have two new super-resolution microscope systemsin the BIRC!
Please contact Alison or Kaye about the super-resolution systems.
- The OMX 3D-SIM system from Applied Precision performs 3D-Structured Illumination Microscopy on samples up to around 20 microns in thickness, labeled with up to 3 conventional dyes or fluorescent proteins, achieving double the normal resolution in all 3 axes (~120nm in x,y and ~300nm in z). The system was recently upgraded to the "Blaze" version, achieving imaging speeds compatible with certain live cell processes.
- The Nikon STORM system uses precision localization technology to achieve up to ~25 nm resolution in xy and 50nm in z over a range of 600nm. The system can be used for 2D or 3D STORM and is compatible with photoswitchable dye pairs or photoswitchable fluorescent proteins.
- We have added a confocal stereology package (Visiopharm) to one of our LSM 510 inverted confocal systems.
Please talk to Kaye about stereology-based assays.
- Two new confocal microscopes have recently been installed in the BIRC - a Zeiss LSM 780 system and a Leica TCS SP5 II system. The Leica system replaced two of our oldest confocal microscopes.
Please ask any of the BIRC staff about the capabilities of the new confocal systems.
- The Olympus multiphoton system has been upgraded to a dual IR-laser system, permitting simultaneous photoactivation or photobleaching using one excitation wavelength and imaging using a different IR excitation wavelength.
Please contact Shiva for more information regarding the multiphoton capabilities of the BIRC.