Usage Rates for Fiscal Year 2014

 

Usage Rates for Fiscal Year 2014

(July 1, 2013 to June 30, 2014)

General

 

RU users ($)

Non-RU users ($)

Consultation

 

FREE

227/hour

Training ᵟ

 

FREE

227/hour

Category I (done by users) *

Expected labor hours for a typical experiment

RU users ($)

Non-RU users ($)

Processing Tissue (manual)

2 days prep  +  2 days resin polymerization

10/run

20/run

Automated sample process

~24 hour

70/run

140/run

Immuno-EM

Widely variable

10/run

20/run

Use of BioWave (Microwave)

N/A

10/hour

20/hour

Use of High Pressure Freezing

N/A

150/run

300/run

Use of Automatic Freeze Substitution

N/A

20/day

40/day

Staining

1 hour

Free

Free

Special Techniques 1: Critical Point Dryer, Ion Spatter, Glow discharge

1hour

10/hour

20/hour

Special Techniques 2: Negative staining, Rotary shadowing, etc.

 1hour

10/hour

20/hour

Ultrathin sections

N/A

10/hour

20/hour

Scoping (TEM - SEM)

N/A

42/hour

84/hour

Miscellaneous supply

N/A

10

20

Category II (done by EMRC staff)

Expected labor hours for a typical experiment

RU users ($)

Non-RU users ($)

Processing Tissue (up to 6 samples/run)

~8 hour

63/hour

126/hour

Immuno-EM (up to 6 samples/run)*

Widely variable

63/hour

126/hour

Bio Wave Process

N/A

63/hour

126/hour

Use of High Pressure Freezing*

N/A

192/run

384/run

Use of Automatic Freeze Substitution (up to 5 days run)*

N/A

96/run

192/run

Staining

< 1 hour

57/hour

114/hour

Special Techniques 1: Critical Point Dryer, Ion Spatter, Glow discharge

< 1 hour

33/hour

66/hour

Special Techniques 2: Negative staining, Rotary shadowing, etc.

< 1 hour

57/hour

114/hour

Ultrathin sections I (for samples prepared by EMRC staff)

< 1 hour

65/hour

130/hour

Ultrathin sections II (for samples prepared by others)

< 1 hour

65/hour

130/hour

Cutting Ultrathin sections III (serial section)

~2 hour

95/hour

190/hour

Cryo-ultrathin section

N/A

95/hour

190/hour

Scoping (TEM /SEM)

N/A

63/hour

126/hour

Image Process/Analysis

N/A

63/hour

126/hour

Miscellaneous labor

N/A

63/hour

126/hour

Miscellaneous supply

N/A

10

20

 

The training fee is applicable only to non-Rockefeller users.  For the users who wish to learn EM procedures, staff will provide training on procedures as they work together with the user on real samples and the overall project will be charged at Category II rates.  Once the user has demonstrated competency, subsequent work is charged at Category I rates.  Because the training is customized to the user’s need, the total time required is variable.

 

* Immuno-EM as well as use of High Pressure freezing or Freeze substitution will be combined with Processing tissue for regular use.

 

Examples of typical experiments: The following examples are provided as guidance for time and budget planning:

1) Conventional EM: discussion (1hr) + tissue process (~8hr) + cutting sections (~1hr/sample) + staining + scope time

2)  Immuno EM: discussion (1hr) + Immuno-staining (~10 hr) + tissue process (~8hr) + cutting sections (30-1hr/sample) + staining + scope time

3) Negative staining: discussion (1hr) + glow discharge (20 min) + negative staining (20 min) + scope time

4) Immuno EM and Negative staining: discussion (1hr) + glow discharge (10 min) + negative staining (20 min) + scope time

5) Training of Negative staining/ Operation of EM: Approximately ~1hr for either training.