Usage Rates for Fiscal Year 2015

 

Usage Rates for Fiscal Year 2015

(July 1, 2014 to June 30, 2015)

General

 

RU users ($)

Non-RU users ($)

Consultation

 

FREE

150/hour

Training *

 

FREE

150/hour

Category I (done by users) *

Expected labor hours for a typical experiment

RU users ($)

Non-RU users ($)

Processing Tissue (manual)

2 days prep  +  2 days resin polymerization

10/run

20/run

Automated sample process

~24 hour

70/run

140/run

Immuno-EM

Widely variable

10/run

20/run

Use of BioWave (Microwave)

N/A

10/hour

20/hour

Use of HPF*

N/A

150/run

300/run

Use of AFS2*

N/A

20/day plus 25 for a charge of LN2

40/day plus 25 for a charge of LN2

Staining

1 hour

Free

Free

Use of device: Critical Point Dry, Ion Spatter, Glow discharge, Glass Knife maker

N/A

10/hour

20/hour

Special Techniques 2: Negative staining, Rotary shadowing

 1hour

10/hour

20/hour

Cryo-EM Preparation (Glow discharge, Vitrobot Plunge, Cryo-Holder Evacuations, LN2)

Variable 

 30/run

60/run 

Ultrathin sections

N/A

10/hour

20/hour

Scoping (TEM - SEM)

N/A

42/hour

84/hour

Scoping TEM (After hours only for qualified users)

N/A 

 30/hour

60/hour 

Scoping (LM) 

N/A 

30/hour 

60/hour 

Image analysis station (IMOD, Chimera, AMIRA, Fiji, Image J, etc) 

 N/A

10/hour 

 20/hour

Miscellaneous supply

N/A

10

20

Category II (done by EMRC staff)

Expected labor hours for a typical experiment

RU users ($)

Non-RU users ($)

Processing Tissue (<6 samples/run)

~8 hour

528/run

1008/run

Immuno-EM (<6 samples/run)*

Widely variable

66/hour

126/hour

Bio Wave (Microwave) Process

N/A

66/hour

126/hour

Use of HPF*

N/A

200/run

400/run

Use of AFS*

N/A

100/run plus 25 for a charge of LN2

200/run plus 25 for a charge of LN2

Staining

< 1 hour

60/hour

120/hour

Special Techniques 1: Critical Point Dryer, Ion Spatter, Glow discharge

< 1 hour

35/hour

70/hour

Special Techniques 2: Negative staining, Rotary shadowing, etc.

< 1 hour

60/hour

120/hour

Ultrathin sections I (for samples prepared by EMRC staff)

< 1 hour

68/hour

136/hour

Ultrathin sections II (for samples prepared by others)

< 1 hour

68/hour

136/hour

Cutting Ultrathin sections III (serial section)

~2 hours

100/hour

200/hour

Cryo-ultrathin section

N/A

100/hour

200/hour

Scoping (TEM /SEM)

N/A

66/hour

132/hour

Image Process/Analysis

N/A

66/hour

132/hour

Miscellaneous labor

N/A

66/hour

132/hour

Miscellaneous supply

N/A

10/unit

20/unit

 

*The training fee is applicable only to non-Rockefeller users.  For the users who wish to learn EM procedures, staff will provide training on procedures as they work together with the user on real samples and the overall project will be charged at Category II rates.  Once the user has demonstrated competency, subsequent work is charged at Category I rates.  Because the training is customized to the user’s need, the total time required is variable.

 

* Immuno-EM as well as use of High Pressure freezing or Freeze substitution will be combined with Processing tissue for regular use.

 

Examples of typical experiments: The following examples are provided as guidance for budget planning:

1. Conventional EM: discussion (1hr) + tissue process (~8hr) + cutting sections (~1hr/sample) + staining + scope time

2.  Immuno EM: discussion (1hr) + Immuno-staining (~10 hr) + tissue process (~8hr) + cutting sections (30min-1hr/sample) + staining + scope time

3. Negative staining: discussion (1hr) + glow discharge (20 min) + negative staining (20 min) + scope time

4. Immuno EM and Negative staining: discussion (1hr) + glow discharge (1 run) + negative staining (30 min-1hr) + scope time

5. Training of operation of EM: Approximately ~1hr of operation training.