Guidelines

To help us maintain the Flow Cytometry Resource Center at its optimum, we ask that you read and follow these guidelines and be considerate of other users. If you have questions or requests, need advice on procedures or have any suggestions or concerns, please do not hesitate to contact FCRC manager at any time.

  1. RESOURCE AVAILABILITY.
    1. Cell Sorters - BD FACSAriaII-1, BD FACSAriaII-2, and BD FACS AriaII-3.
      1. The instruments and operators are available for sorts Monday through Friday according to the Cell Sorting Schedule. After-hour sort time starts at 4:30 pm and should be booked in advance. We cannot guarantee extended hours without advance booking.
      2. The sorts are scheduled on a first-booked-first-served basis.
      3. Cell Sort Scheduling is done at the discretion of the FCRC, to maximize availability for the entire RU community. FCRC Staff schedules sorts from the beginnings or the ends of the day to avoid breaking large time blocks.
      4. If time is unscheduled one day prior to sort, FCRC Staff can adjust the schedule to fit better particular user needs.
      5. There is no time limit for cell sorting (for both - length and time in advance)
      6. To schedule a sort, please:
      7. Prior to the first sort of a new protocol, please arrange an appointment with FCRC manager to discuss the details of the sort.
      8. All the sorts are performed by FCRC operators only.
      9. Condition of NYSTEM funding for the FACSAria II-2:
        • Support through the NYSTEM grant shall be acknowledged in all publications, presentations and products of research resulting from use of or data acquired through the BD FACSAriaII-2, in a form consistent with the publication's guidelines.
        • The acknowledgement should reference "Support by the Empire State Stem Cell fund through NYSDOH Contract #C023046" and include following statement "Opinions expressed here are solely those of the author and do not necessarily reflect those of the Empire State Stem Cell Fund, the NYSDOH, or the State of NY."
        • Please be sure to send to fcrc@rockefeller.edu documentation for publications funded wholly or in part by the contract that include an acknowledgment of this funding support. This information must be submitted to NYSTEM within 60 days of publication.
    2. Imaging Flow Cytometer – ImageStream-X
      1. Sample Acquisition is scheduled on a first-booked-first-served basis.
      2. Sample Acquisition Scheduling is done at the discretion of the FCRC, to maximize availability for the entire RU community. FCRC Staff schedules acquisition from the beginnings or the ends of the day to avoid breaking large time blocks.
      3. If time is unscheduled one day prior to Sample Acquisition, FCRC Staff can adjust the schedule to fit better particular user needs.
      4. There is no time limit for Sample Acquisition (for both - length and time in advance)
      5. To schedule a Sample Acquisitions, please:
      6. Prior to the first Sample Acquisition of a new protocol, please arrange an appointment with FCRC Staff to discuss the details of the sort.
      7. All the Sample Acquisitions are performed by FCRC operators only.
    3. BASIC (BD Accuri® C6) and ADVANCED (BD LSRII-1 and BD LSRII-2) Cell Analyzers.
      1. The Analyzers are available 24/7 except during maintenance periods (see Section B. Weekly Maintenance below)
      2. Data acquisition on the Flow Cytometry Cell Analyzers is done by researchers and considered as self-service
      3. FCRC-PPMS System allows users to book time on the Cell Analyzers:
        • Time could be booked up to two weeks in advance
        • The experiments are scheduled on a first-booked-first-served basis
        • You may schedule in 30-minute increments and generally should not book more than 2 hours at a time during the high demand period (weekdays: 8:00 am - 10:00 pm)
        • If your procedure requires more than 2 hours, please sign-up for non-peak hours (when there is no maximum time restriction) or contact FCRC staff to book time for you
      4. During the acquisition, the data files could be saved in the temporary folder on the desktop of the computer attached to the instrument. After the completion of the experiment, all the files should be moved out (Refer to Computing — BD LSRII Data Management and Data Storage and Retention Policy).
      5. FCRC is not responsible for data files left on FCRC computers.
      6. All the experiments are performed by researchers themselves unless FCRC personnel assistance is requested.
      7. To get an appropriate assistance from FCRC staff please be sure to schedule it in advance by contacting FCRC staff
      8. FCRC staff assistance is free of charge for RU researchers (see Charges and Fees)
    4. Analysis Computers - FCRC MAC Analysis I, FCRC MAC Analysis II, FCRC Analysis PC1, and FCRC Analysis PC2(other than these attached to the instruments above).
      1. The computers are available 24/7
      2. Access to the computers is on a first-come-first-served basis (without signup).
      3. These computers are dedicated to flow cytometry data analysis only. They are on the internet, run Flow Cytometry (CellQuest Pro, FlowJo, BD FACSDiVa, FCS Express, ModFit) and Office programs to analyze and publish data.
      4. Absolutely no data can be stored on the FCRC MAC Analysis I, FCRC MAC Analysis II, and FCRC Analysis PC1 machines. FCRC Analysis PC2 is dedicated for ImageStream-X Data analysis, and data should be saved in this computer.
      5. After the completion of the experiment, all the files should be moved out (Refer to Computing — BD LSRII Data Management and Data Storage and Retention Policy).
      6. All the data analysis is performed by researchers themselves unless FCRC personnel assistance is requested.
      7. To get an appropriate assistance from FCRC staff please be sure to schedule it in advance by contacting FCRC staff
      8. FCRC staff assistance is free of charge for RU researchers (see Charges and Fees)
  2. WEEKLY MAINTENANCE.
    1. Regular weekly maintenance on all FCRC Analyzers is scheduled on Mondays (see time blocks on FCRC-PPMS System).
    2. Regular weekly maintenance includes the following procedures on all FCRC Analyzers
      1. Instrument cleaning according to BD-recommended procedures for Weekly or Monthly Maintenance
      2. Quality Control according to BD-recommended procedures
      3. Fresh preparation of all the solutions for spray and drop bottles
      4. Removal and discard of all unlabeled sample tubes and racks from the desks and benches
      5. Purge of all illegitimate files from the computers
  3. CHARGES AND CANCELLATION POLICY.
    1. To recover essential operating costs, the FCRC charges hourly fees for the sorts and use of the analyzers according to Charges and Fees.
    2. FCRC staff assistance and usage of computers (other than those attached to the instruments) is free of charge.
    3. To avoid cancellation fees, scheduled operator-assisted services (BD FACSAria-1, BD FACSAria-2, BD FACSAria-3 and ImageStream-X) must be cancelled no later than 5 pm two business days prior to the scheduled sort. If the scheduled sort is not cancelled by this time, the FCRC will impose a charge for one-half of total time originally scheduled. For details please refer to Charges and Fees.
    4. Scheduled use of the Analyzers must be cancelled no later than two hours in advance of the scheduled start time. If the scheduled use of the analyzer is not cancelled by this time, the FCRC will impose a charge for one-half of the original amount of time scheduled.
    5. Charges on Cell Analyzers are calculated based on logged computer time, using "login/logout" report.
  4. LOGIN SYSTEM.
    1. Please read detailed information about Login System before planning your experiment.
    2. Be sure to set up all necessary logins/passwords in advance according to Login and Flow Cytometry Class Requirements.
    3. Please use your own login ID only and log off at the earliest convenience to avoid extra charges.
  5. OPERATION PROCEDURES.
    1. All FCRC Analyzers are run in combination with BD FACSFlow Supply Systems attached to the instruments. To avoid instrument malfunction be sure to read and follow the Maintenance Guidelines.
    2. Each user is responsible to wash instrument after sample acquisition is completed. Detailed notes on washing procedures are posted on the instruments. After instrument is washed, be sure to put it on "standby".
    3. During regular business hours (9:00 am - 6:00 pm on weekdays) please leave Instrument "on".
    4. During weekends and off hours (after 6:00 pm on weekdays and all day on weekends and holidays) please switch "off" Instrument.
    5. Do not forget to Log off from FCRC computers after work completed.
    6. Never turn "off" FCRC computers to allow remote access for RU IT and scheduled backups to be performed.
    7. Clean up FCRC bench space and discard all the tubes into the red containers for biohazard waste.
    8. Please discard all non-biohazard waste into black containers for regular waste.
  6. TRAINING, CONSULTATION, DISCUSSION AND TROUBLESHOOTING.
    1. The subjects reviewed at the training sessions vary depending on the background, needs and interests of the investigators. See Flow Cytometry Class Requirements. Please do not hesitate to contact FCRC manager to find out the best solution for you.
    2. For the Basic, Advanced, HTS hands-on, and Accuri® C6 hands-on training sessions please fill up Flow Cytometry Class Application. Training sessions are scheduled on a first-booked-first-served basis.
    3. For consultation, discussion and troubleshooting, feel free to contact the FCRC Manager during regular working hours or 24/7 via e-mail.
  7. THE FACILITY.
    1. The FCRC is located in rooms 205, 207 and 211 of the Detlev W. Bronk Building (DWB 205, 207 and 211).
    2. The FCRC door is unlocked from 9:00 am to 6:00 pm, Monday to Friday. During weekends and off hours, access to the facility is limited to those with authorized University ID cards.
    3. You may have your ID card programmed for access by requesting RU Card Activation for FCRC Door Access. For safety purposes, please do not prop open the door to the FCRC.