DNA/RNA Sample Preparation and Quality Control

It's very important that users provide us DNA or RNA samples at required quality and quantity. Please refer to the Services section for specific requirements of sample submission. Here are some general guidelines for sample preparation and quality control:

Total RNA preparation

RNA quantitation

We recommend the NanoDrop spectrophotomer for quick and accurate quantitation. NanoDrop at our center is available to you free of charge.

RNA quality assessment

The quality of total RNA samples is very important in microarray experiments. When you provide us total RNA samples for labeling, we always perform RNA integrity check by running RNA samples on Agilent 2100 Bioanalyzer. If there is significant degradation (when RNA Integrality Number, or RIN, is below 8.0), we will NOT perform sample labeling. Instead, we will request users re-submit total RNA samples.

If you perform sample labeling yourself, we highly recommend that you also perform RNA integrity analysis first.

Genomic DNA Preparation for SNP genotyping and sequencing

DNA quantitation

We recommend the NanoDrop spectrophotomer for quick and accurate quantitation. NanoDrop at our center is available to you free of charge.

Genomic DNA Quality Assessment: