High-Throughput and Spectroscopy Resource Center
High-Throughput Screening (HTS) requires the miniaturization and automation of bioassays so that millions of variables can be tested and is often an important step in the discovery of new medicines. The High-Throughput and Spectroscopy Resource Center (HTSRC) supports researchers in improving the efficiency of their bioassays, identifying compounds and genetic modulators of function, and in utilizing core technologies typically applied to biochemical analysis. The center has a collection of 185,000 compounds, automated liquid transfer devices, compound databases, and supports a broad diversity of assay development techniques, typically found in early drug discovery programs.
The Center is configured for processing microtiter plates through the use of automated changeable tip dispensers, non-contact dispensers, and syringe dispensers. For assay technologies, the HTSRC has the capability to support cellular and biochemical assays using absorbance, fluorescent kinetics, fluorescence anisotropy,time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, AlphaScreen, bioluminescence, for example luciferase and green fluorescent protein, scintillation proximity and cellular fluorescence imaging. Assay targets can include ion channels, receptors, enzymes, protein interactions, signaling pathways and cellular processes.
The Center also supports spectroscopic and calorimetric equipment for use in studies of the structure, function and interactions of biological and organic molecules. The following instruments and associated techniques are available: a microscale thermophoresis instrument, a circular dichroism spectrometer (CD), a surface plasmon resonance detector (SPR), an isothermal calorimeter (ITC) and 2 NMRs.
On a collaborative basis, the HTSRC can support projects from academic institutions outside of Rockefeller or Weill-Cornell Medical College; however, the HTSRC does not currently support research in conjunction with for-profit entities.