Services & Training
The CBSRC allows independent use of its equipment by those qualified, as well as extensive training and familiarization for those less-experienced in spectroscopic methods. Usage and training are by arrangement with the center's manager, Yufeng Wei, Ph.D.
In addition to instrumentation, the center also offers such practical amenities as a cold room, research benches and a small reference library.
1D Proton NMR on Bruker DPX400: a demo session will be held periodically every month. People who are interested in an introductorysession in running NMR experiments can sign up in advance with the Facility Manager. A person can also be trained by an existing NMR user for this session. All new users must complete a 'check out' procedure with the Facility Manager, Yufeng Wei, before he or she can operate the spectrometer on their own.
Included in this session: login/logout, starting and exiting Xwinnmr, locking, shimming, data acquition, data processing, referencing, pick picking, integration, and plotting.
Carbon and heteronuclear NMR on Bruker DPX400: a demo session will be scheduled upon request. All users interested in running 13C, 15N, 31P experiments should contact Yufeng Wei for a demo appointment. Included in this session: probe tuning and matching, qualitative spectra, J(C-H) coupling measurement, DEPT experiments.
2D and advanced NMR on Bruker DPX400: All users interested in acquiring two-dimensional NMR data and other advanced experiments should contact Yufeng Wei for a demo appointment. This includes any user who will perform the following experiments: gradient NMR, water suppresion, two dimensional experiments (COSY, NOESY, HMQC, HSQC, HMBC).
A user guide for each training session will be distributed during the demo and is available on the web site (PDF format).
All new users should make arrangements with Yufeng Wei to watch him or her to take and process a spectrum. An individual can only operate the spectrometer after he or she has successfully completed the 'check out' procedure.
Check out includes the following:
- login/logout, starting and exiting Xwinnmr
- locking and shimmimg
- acquisition and processing
- referencing, peak picking, and integration
13C/15N/31P must pass 'check out' for probe tuning/matching, and 1D heteronuclear experiments.
Advanced users must pass all previous 'check out', and get trained on the experiments they want to run.
Users are responsible for backing up their own data generated from optical spectrometers, including the Biacore SPR, CD, IR, Fluorimeter, and Phospho Imager, immediately after their experiments on these instruments. Data are not retained on these instruments, and are subject to deletion to make disk space available for new experiments.
For NMR use, each user is given an individual account. Users must login to their own account to use the instrument. Do NOT use other user's account. If somebody forgets to logout, the next user should log the other person out, and login to his or her account before running any experiments.
NMR data from both 400 and 600 MHz spectrometers will be retained for up to 3 months. Any NMR data older than 3 months are subject to deletion. Users will have to contact IT directly for retrieving NMR data older than 3 months from IT managed backups. The SRC makes no guarantees of retrievability of NMR data older than 3 months. It is suggested that users backup their own NMR data immediately after their experiments, but no later than 3 months after generating the data. A secure FTP client, such as WinSCP and Fetch, is recommended for the purpose of backing up NMR data. Users can establish a secure FTP connection to mr600.rockefeller.edu and dpx400a.rockefeller.edu for 600 and 400 MHz spectrometers, respectively, and log in using their own usernames and passwords. A secure FTP connection can only be established on campus network or via VPN. Users' data are located at /opt/topspin/data/username/nmr (replacing username with your login name) on either instrument. For further information and assistance on secure FTP, please contact IT.