Antibody Discovery
The facility works with researchers to generate custom MAbs by offering:
- Comprehensive project design and management (free).
- Immunization of mice, rats or hamsters with peptides, protein, cells and DNA.
- Creating hybridomas / stable MAb secreting cell lines via electrofusion, generating 10’s of thousands hybridomas for screening.
- Maintenance of the newly immortalized hybridomas during the screening process.
- Screening new hybridoma cultures / MAbs, specifically using ELISA, flow cytometry, immunofluorescence, and western blot.
- Cryopreservation and repository for positive hybridoma cultures.
- Subcloning hybridoma populations that recognize the antigen of interest to create stable hybridoma stocks.
- Oversight of the validation process for free.
For more information, contact Frances at 646-888-2354 or weisgar@rockefeller.edu / weisgarf@mskcc.org.