Antibody Discovery

The facility works with researchers to generate custom MAbs by offering:

• Comprehensive project design and management (free).
• Immunization of mice, rats or hamsters with peptides, protein, cells and DNA.
• Creating hybridomas / stable MAb secreting cell lines via electrofusion, generating 10’s of thousands hybridomas for screening.
• Maintenance of the newly immortalized hybridomas during the screening process.
• Screening new hybridoma cultures / MAbs, specifically using ELISA, flow cytometry, immunofluorescence, and western blot.
• Cryopreservation and repository for positive hybridoma cultures.
• Subcloning hybridoma populations that recognize the antigen of interest to create stable hybridoma stocks.
• Oversight of the validation process for free.

For more information, contact Frances at 646-888-2354 or weisgar@rockefeller.edu / weisgarf@mskcc.org.