The rockefeller university

In 2017 the Center included the analysis of lipids and metabolites in the tools offered. The Center analyzes samples using electrospray(opens in new window) based high resolution high mass accuracy (HRMS) LC-MS/MS  Orbitrap mass spectrometers coupled to higher flow liquid chromatography. Both reversed phase(opens in new window) and hydrophilic interaction liquid chromatography(opens in new window) (HILIC)  is used.

The Center has experience with targeted as well as un-targeted analysis of small molecules, polar metabolites and lipids and are also capable of measuring pulse/chase studies based on stable heavy isotopes.  Typical projects analyzed by the Center includes:

• Small drugs ¹(opens in new window).
• Polar metabolites ²(opens in new window).
• Nucleosides ³(opens in new window).
• Data Dependent lipid profiling ⁴(opens in new window).
• Aldehydes analysis based on derivation ⁵(opens in new window).

References

1. Bellat, V. et al. Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme-Induced Local Retention Properties. Advanced functional materials 28 (2018).
2. Garcia-Bermudez, J. et al. Aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours. Nature cell biology 20, 775-781 (2018).
3. Westcott, N.P., Fernandez, J.P., Molina, H. & Hang, H.C. Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. Nat Chem Biol (2017).
4. Zhu, X.G. et al. CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4. Mol Cell (2019).
5. Matsumura, Y. et al. Gene Therapy Correction of Aldehyde Dehydrogenase 2 Deficiency. Molecular therapy. Methods & clinical development 15, 72-82 (2019).