Metabolomics/Lipidomics
In 2017 the Center included the analysis of lipids and metabolites in the tools offered. The Center analyzes samples using electrospray based high resolution high mass accuracy (HRMS) LC-MS/MS Orbitrap mass spectrometers coupled to higher flow liquid chromatography. Both reversed phase and hydrophilic interaction liquid chromatography (HILIC) is used.
The Center has experience with targeted as well as un-targeted analysis of small molecules, polar metabolites and lipids and are also capable of measuring pulse/chase studies based on stable heavy isotopes. Typical projects analyzed by the Center includes:
- Analysis of small drugs 1.
- Analysis of polar metabolites 2.
- Nucleoside analysis 3.
- Data Dependent lipid profiling 4.
- Aldehyde analysis based on derivation 5.
References
- Bellat, V. et al. Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme-Induced Local Retention Properties. Advanced functional materials 28 (2018).
- Garcia-Bermudez, J. et al. Aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours. Nature cell biology 20, 775-781 (2018).
- Westcott, N.P., Fernandez, J.P., Molina, H. & Hang, H.C. Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. Nat Chem Biol (2017).
- Zhu, X.G. et al. CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4. Mol Cell (2019).
- Matsumura, Y. et al. Gene Therapy Correction of Aldehyde Dehydrogenase 2 Deficiency. Molecular therapy. Methods & clinical development 15, 72-82 (2019).