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Rockefeller University

Metabolomics/Lipidomics

In 2017 the Center included the analysis of lipids and metabolites in the tools offered. The Center analyzes samples using electrospray based high resolution high mass accuracy (HRMS) LC-MS/MS  Orbitrap mass spectrometers coupled to higher flow liquid chromatography. Both reversed phase and hydrophilic interaction liquid chromatography (HILIC)  is used.

The Center has experience with targeted as well as un-targeted analysis of small molecules, polar metabolites and lipids and are also capable of measuring pulse/chase studies based on stable heavy isotopes.  Typical projects analyzed by the Center includes:

  • Analysis of small drugs 1.
  • Analysis of polar metabolites 2.
  • Nucleoside analysis 3.
  • Data Dependent lipid profiling 4.
  • Aldehyde analysis based on derivation 5.

 

 

 

 

 

 

References

  1. Bellat, V. et al. Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme-Induced Local Retention Properties. Advanced functional materials 28 (2018).
  2. Garcia-Bermudez, J. et al. Aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours. Nature cell biology 20, 775-781 (2018).
  3. Westcott, N.P., Fernandez, J.P., Molina, H. & Hang, H.C. Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. Nat Chem Biol (2017).
  4. Zhu, X.G. et al. CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4. Mol Cell (2019).
  5. Matsumura, Y. et al. Gene Therapy Correction of Aldehyde Dehydrogenase 2 Deficiency. Molecular therapy. Methods & clinical development 15, 72-82 (2019).

 

 

Internal Rockefeller users can access pLIMS sample information, progress, and final invoices using the link below.
Sample Status

Contact

The Rockefeller University
The Proteomics Resource Center, Box 105
1230 York Avenue
New York, NY 10065-6399