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The rockefeller university

Instruments

 

Pacific Biosciences Sequels

Image of Pac BIo

The RGRC has two PacBio Sequel 2 instruments and one PacBio Sequel 1 instrument. The PacBio Sequel is a third-generation sequencer based on PacBio-proven Single Molecule Real-Time (SMRT) technology to generate long reads with high consensus accuracy and uniform coverage.

Applications:

  • Reference genome assembly (CLR or Hifi)
  • Full length transcriptome sequencing (Iso-seq)
  • Complex populations (e.g. bacteria, viruses)
  • Targeted sequencing
  • Epigenetic

Bionano Genomics Saphyr

The Saphyr System

The Saphyr System is an optical mapping automated instrument. It allows imaging of high-molecular weight DNA migrating through NanoChannel arrays on the Saphyr Chip. This technology can be used to study of genomic structural variation sensitivity and scaffolding genomes.

Applications:

  • Structural variation (large insertions/deletions, translocations, inversions)
  • Reference genome assembly (scaffolding and assemblies correction)