The rockefeller university

 Overview(opens in new window) – 5:01(opens in new window)

• Define the term flow cytometry.
• Describe the types of samples suitable for a flow cytometer.
• Explain the properties that a flow cytometer can measure.
• Identify the three subsystems of a flow cytometer.

 Optical measurements – 9:48(opens in new window)

• Describe the factors that affect light scatter.
• Explain how light scatter and fluorescence can be used to detect populations.
• Describe the use of excitation and emission spectra.
• Explain what compensation is and why it is necessary for multicolor analysis.

 Sorting – 4:25(opens in new window)

• Describe the purpose of sorting cells or particles.
• Explain how particle size affects the nozzle size and sheath pressure selection.
• Describe the steps in droplet sorting.

Optimize your Flow Cytometry-Best Practices for Sample Preparation, Staining and Analysis(opens in new window)