The rockefeller university

Overview – 5:01

• Define the term flow cytometry.
• Describe the types of samples suitable for a flow cytometer.
• Explain the properties that a flow cytometer can measure.
• Identify the three subsystems of a flow cytometer.

Optical measurements – 9:48

• Describe the factors that affect light scatter.
• Explain how light scatter and fluorescence can be used to detect populations.
• Describe the use of excitation and emission spectra.
• Explain what compensation is and why it is necessary for multicolor analysis.

Sorting – 4:25

• Describe the purpose of sorting cells or particles.
• Explain how particle size affects the nozzle size and sheath pressure selection.
• Describe the steps in droplet sorting.

Optimize your Flow Cytometry-Best Practices for Sample Preparation, Staining and Analysis

• Overview                                                 1:50 – 2:31
• Sample Preparation                              2:32 – 16:40
• Staining Consideration                       16:41 – 27:38
• Dead Cell Discrimination                    27:39 – 31:11
• Doublet Exclusion                                31:12 – 33:33
• Sample Evaluation                               33:34 – 34:46
• Example of the Workflow                   34:47 – 38:04
• Additional Info and Summary            38:05 – 39:57

Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry. Andrew Reichard, Kewal Asosingh. Cytometry A. 2019 Feb;95(2):219-226