The rockefeller university

Panel Design Step 1: Marker Expression and Co-expression – 8:01

Panel Design Step 2: Fluorochrome Selection – 10:27

Panel Design Step 3: Spillover-Spreading Error -7:30

Panel Design Step 4: Designing a Panel – 10:47

Panel Design Step 5: Evaluate Panel Performance – 7:11

Webinar 20211130 – Autofluorescence Characterization Using Full Spectrum Profiling™ (FSP™) by Kate Pilkington – 41:32

Webinar 20210325 – Just Because You Don’t See It Doesn’t Mean It Isn’t There: Improved Assay Resolution with Autofluorescence Extraction by Alexis Gonzalez – 44:50

Reagent / Antibody titration:

The Power of Reagent Titration in Flow Cytometry. Diana LB, Alberta P, Jesus G, Lily MP, Maria CJ. Cells. 2024 Oct 11;13(20):1677

OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood. Park LM, Lannigan J, Jaimes MC. Cytometry A. 2020 Oct;97(10):1044-1051

OMIP-069 Version 2: Update to the 40-color full Spectrum flow cytometry panel for deep immunophenotyping of major cell subsets in human peripheral blood. Park LM, Lannigan J, Low Q, Jaimes MC, Bonilla DL. Cytometry A. 2024 Nov; 105(11):791-799

OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells. Andrew JK, Peter LM, Aaron JT, Martin P, Florian M. Cytometry A. 2024 Jun;105(6):430-436

OMIP-109: 45-color full spectrum flow cytometry panel for deep immunophenotyping of the major lineages present in human peripheral blood mononuclear cells with emphasis on the T cell memory compartment. Lily MP, Joanne L, Quentin L, Maria CJ, Diana LB. Cytometry A. 2024 Nov;105(11):807-815

Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry. Laura Ferrer-Font, Sam J Small, etc. Current Protocols. 2021 Sep;1(9):e222

Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry. Laura Ferrer-Font, Sam J Small, etc. Current Protocols. 2021 Sep;1(9):e222 – PDF

Additional Resources:

Plan Your Assay

      Sign Up

• • Free Cytek Cloud account to access all spectral panel design tools
  • Aurora User Forum (and look in the Application sub-forum to find Normalization spreadsheets, configuration charts, and more!)

      Panel Design

• • Watch a video series on panel design in Cytek® SpectroLearn™
  • Publication about Panel Design Best Practices
  • Webinar: Building Panels for Flow Cytometry: Key Steps for Success
  • OMIP-069 Cytek’s 40 Color Panel publication – A comprehensive resource for designing and optimizing high parameter panels on Cytek Instruments (Don’t forget to grab the supplement too!)

      Reference Controls

• • Review best practices in the Reference Control video in Cytek® SpectroLearn™
  • List of tandem and non-tandem dyes (attached)

      Titration

• • Webinar on Debunking Antibody Titration Myths
  • Antibody Titration Protocol
  • Blog article by Laura Johnston about the vital importance of properly titrating viability dyes

Run Your Assay

Check out the collection of video tutorials about SpectroFlo if you want more in depth detail about specific features, including:

• • System Startup & QC
  • Create and Unmix an Experiment
  • Plate loaders: AMS loader and ASL loader (newer version)
  • Note that we release frequent software updates so please refer to your TAS or applicationsupport@cytekbio.com for the most up-to-date advice

Gating reference controls – another great blog article by Laura Johnston about setting up gates in the unmixing wizard

Webinar on instrument standardization

 Evaluate Your Assay

• • Publication about Panel Optimization Best Practices
  • Cytek White Paper about Evaluating Panel Performance

Autofluorescence

For more detailed information on AF, check out these webinars:

• • The Power of Autofluorescence Extraction Using Full Spectrum Profiling™
  • Autofluorescence Characterization Using Full Spectrum Profiling™ (FSP™)
  • Full Spectrum Profiling™ (FSP™) Enhances Resolution of Samples with Complex Autofluorescence