Sample Preparation Guidelines
The Center has and continues to develop guidelines for various sample types. The guidelines are not Standard Operating Procedures but meant to give the users an idea about how to prepare samples prior to drop off. Some of the guidelines are very specific while others are general. The guidelines are often a concentrate of our users’ successful experiments. Guidelines are updated as we learn new things and and more will be added when possible.
- Lysis of cells for label-free proteomics experiments.
- Affinity purifications.
- Biotin-streptavidin based purifications.
- Use of the Typhoon Scanner.
- Detailed description of simple gel band digestion.
- HLA1-type analysis.
- Working with Detergents.