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In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling of mutant cells expressing transgenic elements from precisely defined chromosomal loci. We provide a toolkit of MADR elements for combination labeling, inducible and reversible transgene manipulation, VCre recombinase expression, and transgenesis of human cells. Further, we demonstrate the versatility of MADR by creating glioma models with mixed reporter-identified zygosity or with "personalized" driver mutations from pediatric glioma. MADR is extensible to thousands of existing mouse lines, providing a flexible platform to democratize the generation of somatic mosaic mice

Voltage-gated potassium channels (K(v)s) are gated by transmembrane

IgG antibodies are secreted from B cells and bind to a variety of

Background: ATP1A3 encodes the a 3 subunit of the Na+/K+ ATPase, a

Autosomal recessive IRF7 and IRF9 deficiencies impair type I and III IFN

Vaccination is an effective strategy to control influenza disease.

Post-transcriptional regulation of RNA stability is a key step in gene

Although fate maps of early embryos exist for nearly all model

Stochastic mechanisms diversify cell fate in organisms ranging from

In bacteria, a primary a-factor associates with the core RNA polymerase