Reagent Requests & Protocols
Vice President and Chief Scientific Officer, Howard Hughes Medical Institute
Updated February 24, 2024
Reagent Requests
The Vosshall Lab freely shares all published reagents.
Fly stocks, DNA clones, vectors, and antibody reagents can be requested from the Vosshall Laboratory by sending an email to Alexandra DeFoe.
Please note that unless you supply a FEDEX number, all reagent requests will be sent by regular mail. Please supply a complete shipping address and telephone contact number suitable for Fedex delivery (e.g. no PO boxes).
The very popular pCasperAUG-Gal4-X vector for cloning Drosophila enhancer/promoters in front of the Gal4 gene is available directly from Addgene. Addgene is also distributing a growing number of our historic Drosophila OR and GR plasmids as well as newer mosquito gene targeting vectors and mosquito cDNAs: Vosshall Lab Plasmid Collection at Addgene
Many of our published fly strains have been deposited in the Bloomington Drosophila Stock Center. This includes all OR-Gal4 transgenes, and Orco and Gr63a mutant reagents, as well as various tagged UAS-OR constructs. If you are having problems finding our stocks at Bloomington, please download their most current stock list and search for “Vosshall”.
Several of our published Aedes aegypti mutant strains can be ordered from the CDC MR4 resource in Atlanta, GA via BEI Resources. You must register with BEI Resources prior to ordering our live vector mosquitoes.
Protocols
Vosshall Lab Graphic Design Standards for Figures (7/20/2018)
Think your lab is happy? — ASK THEM!!! The Anonymous Lab Survey (September 2019)
Mentoring Compact for Graduate Students and Postdocs in the Vosshall Laboratory (December 21, 2016)
Statement on Authorship Policies in the Vosshall Laboratory (February 14, 2020)
Genome-engineering with CRISPR-Cas9 in the mosquito Aedes aegypti.
Bench Manual for Precision Genome Editing in the Mosquito
Historic Protocols 1987-2000
(from the before times when we didn’t use kits to do everything…)
Whole Mount Fluorescent In Situ Hybridization for Drosophila Larval Brains
Enhanced Two-Color Fluorescent In Situ Hybridization for Low-Abundance Genes
Click here for the complete list of DOR proteins in Vosshall et al.,1999; Vosshall et al., 2000
Preparation of Tissue for Larval In Situ Hybridization (Frozen Sections)
Single Fly Genomic DNA Protocol
ABI Prism 310 Cycle Sequencing Protocol
ABI Prism 310 Cycle Sequencing Protocol – Economy Version
Drosophila Embryo Microinjection
Whole Mount Drosophila Embryo In Situ Hybridization with RNA probes
Whole Mount Fluorescent Antibody Staining of Drosophila Embryos
Expand High Fidelity PCR Reactions
Fixing Embryos for In Situ Hybridization
Making FIXATIVE for In Situ and Antibody Staining
Drosophila Genomic DNA Preparation
Hanahan Competent Cell Protocol
Screening Bacteriophage Lambda Libraries
Whole Mount Fluorescent Antibody Staining of Drosophila Larvae
Mass Dechorionation for Embryo Microinjection
Pharmacia Spin Column Protocol
Stocker Fly Brain Immunofluorescence Protocol
DIG In Situ Hybridization Protocol(with detergent)
X-GAL STAINING [Frozen Cryostat Sections]
X-GAL STAINING [Adult Head Whole Mounts]
Annotation, localization, and clone information for all DOR genes (Excel file for download) (PDF)
Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation