Guidelines for Submitting Transgenic DNA Vector

The following procedure is for Plasmid DNA only. For BAC transgenic construct please contact GTF (x8650) for special instructions.

To submit the DNA for pronuclear injection please prepare the following materials:

1. 50ug of transgenic construct (insert plus vector) that has been digested with RE to remove vector.
2. A picture of the DNA gel to demonstrate the completion of RE digestion. Please indicate clearly on the picture which DNA fragment should be purified for injection.
3. A map of your construct with your contact information.
4. Please indicate on which mouse background the transgenic production should be.

GTF will purify the insert DNA for you.

You can contact GTF by phone at x8650.